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Human skeletal muscle carnitine palmitoyltransferase I activity determined in isolated intact mitochondria.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Berthon, P. M. Howlett, Richard A. Heigenhauser, George J. F. Spriet, Lawrence L. |
| Copyright Year | 1998 |
| Abstract | This study was designed to compare the activity of skeletal muscle carnitine palmitoyltransferase I (CPT I) in trained and inactive men (n = 14) and women (n = 12). CPT I activity was measured in intact mitochondria, isolated from needle biopsy vastus lateralis muscle samples ( approximately 60 mg). The variability of CPT I activity determined on two biopsy samples from the same leg on the same day was 4.4, whereas it was 7.0% on two biopsy samples from the same leg on different days. The method was sensitive to the CPT I inhibitor malonyl-CoA (88% inhibition) and therefore specific for CPT I activity. The mean CPT I activity for all 26 subjects was 141.1 +/- 10.6 micromol . min-1 . kg wet muscle (wm)-1 and was not different when all men vs. all women (140.5 +/- 15.7 and 142.2 +/- 14.5 micromol . min-1 . kg wm-1, respectively) were compared. However, CPT I activity was significantly higher in trained vs. inactive subjects for both men (176.2 +/- 21.1 vs. 104.1 +/- 13.6 micromol . min-1 . kg wm-1) and women (167.6 +/- 14.1 vs. 91.2 +/- 9.5 micromol . min-1 . kg wm-1). CPT I activity was also significantly correlated with citrate synthase activity (all subjects, r = 0.76) and maximal oxygen consumption expressed in milliliters per kilogram per minute (all subjects, r = 0.69). The results of this study suggest that CPT I activity can be accurately and reliably measured in intact mitochondria isolated from human muscle biopsy samples. CPT I activity was not affected by gender, and higher activities in aerobically trained subjects appeared to be the result of increased mitochondrial content in both men and women. |
| File Format | PDF HTM / HTML |
| DOI | 10.1152/jappl.1998.85.1.148 |
| PubMed reference number | 9655768 |
| Journal | Medline |
| Volume Number | 85 |
| Issue Number | 1 |
| Alternate Webpage(s) | http://jap.physiology.org/content/jap/85/1/148.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1152/jappl.1998.85.1.148 |
| Journal | Journal of applied physiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
