Isolation of the Arabidopsis ABI3 gene by positional cloning.

Content Provider	Semantic Scholar
Author	Giraudat, Jérôme Hauge, Brian M. Valon, Christiane Smalle, Jan Parcy, François Goodman, H. M.
Copyright Year	1992
Abstract	Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The ABI3 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region was constructed. An RFLP marker closely linked to the abi3 locus was identified, and by analyzing an overlapping set of cosmid clones containing this marker, the abi3 locus was localized within a 35-kb region. An 11-kb subfragment was then shown to complement the mutant phenotype in transgenic plants, thereby further delimiting the position of the locus. A candidate ABI3 gene was identified within this fragment as being expressed in developing fruits. The primary structure of the encoded protein was deduced from sequence analysis of a corresponding cDNA clone. In the most severe abi3-4 allele, the size of this predicted protein was reduced by 40% due to the presence of a point mutation that introduced a premature stop codon. The predicted ABI3 protein displays discrete regions of high similarity to the maize viviparous-1 protein.
Starting Page	1824
Ending Page	1829
Page Count	6
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.plantcell.org/content/plantcell/4/10/1251.full.pdf
PubMed reference number	1359917v1
Volume Number	4
Issue Number	10
Journal	The Plant cell
Language	English
Access Restriction	Open
Subject Keyword	ABI3 gene Abscisic Acid Clone Cells Cloning Vectors Codon Genus Codon, Nonsense Complement System Proteins Cosmids DNA, Complementary Fruit Germination Plants, Transgenic Point Mutation Restriction fragment length polymorphism Sequence Analysis mutant seed development
Content Type	Text
Resource Type	Article