Plant protein hydrolysates from soybean and rice grain as a supplement for medium in human skin fibroblast 1184 cell culture

Content Provider	Semantic Scholar
Author	Farizhandi, Amir Abbas Kazemzadeh Rahman, Sharifah Annirah Syed Abd Zaidel, Dayang Norulfairuz Abang Muhamad, Ida Idayu Majid, Fadzilah Adibah Abdul
Copyright Year	2014
Abstract	In cell culture, addition of serum in the medium plays an important role for the growth of the cells. Each medium consists of two main parts which are a basal media and serum. Serum, mainly from animal source is very expensive and possesses higher potential of viral contamination. The alternative for solving this problem is to use a serum-free media with an addition of some supplements. The most important source of supplement is from plants which could reduce the cost. In this study, protein hydrolysates from soybean and rice as a supplement for medium in human skin fibroblast (HSF) 1184 cells to substitute the addition of serum in the medium were investigated. Plant protein hydrolysates from soybean and rice were prepared through enzymatic hydrolysis using two different enzymes; Alcalase and Flavourzyme. These hydrolysates were characterized according to their solubility and peptide size. Different growth behavior was observed when these protein hydrolysates were added in medium with and without Fetal Bovine Serum (FBS). Protein hydrolysates produced using Flavourzyme has no significant effect on cell culture, while hydrolysates from Alcalase were supplementary for HSF 1184 cell culture. Since plant proteins do not contain all the necessary amino acids for HSF 1184 cell culture growth, they cannot be solely substituted with FBS. Depending on the enzyme used, supplementation with hydrolysates corresponding to a high degree of hydrolysis and composition of peptides with small molecular size, led to different maximal cell density. It is whereby degree of hydrolysis for soybean and rice hydrolysed by Alcalaseis slightly higher (31.59% and 36.52% for soybean and rice, respectively) when compared to hydrolysis by Flavourzyme (17.58% and 34.36% for soybean and rice, respectively). Results also demonstrated that preparation procedure for protein isolates were efficient because the protein concentration was about two times greater than primary meal and some undesirable materials for mammalian cell culture were eliminated from meal. From this study, protein hydrolysates from soybean hydrolysed by Alcalase have shown the best performance for supplementation into a medium for HSF 1184 cell growth.
Starting Page	838
Ending Page	843
Page Count	6
File Format	PDF HTM / HTML
Volume Number	8
Alternate Webpage(s)	http://www.aensiweb.com/old/aeb/2014/838-843.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article