The specification of telencephalic glutamatergic neurons from human pluripotent stem cells.

Content Provider	Semantic Scholar
Author	Boisvert, Erin M. Denton, Kyle R. Lei, Ling Li, Xue-Jun
Copyright Year	2013
Abstract	Here, a stepwise procedure for efficiently generating telencephalic glutamatergic neurons from human pluripotent stem cells (PSCs) has been described. The differentiation process is initiated by breaking the human PSCs into clumps which round up to form aggregates when the cells are placed in a suspension culture. The aggregates are then grown in hESC medium from days 1-4 to allow for spontaneous differentiation. During this time, the cells have the capacity to become any of the three germ layers. From days 5-8, the cells are placed in a neural induction medium to push them into the neural lineage. Around day 8, the cells are allowed to attach onto 6 well plates and differentiate during which time the neuroepithelial cells form. These neuroepithelial cells can be isolated at day 17. The cells can then be kept as neurospheres until they are ready to be plated onto coverslips. Using a basic medium without any caudalizing factors, neuroepithelial cells are specified into telencephalic precursors, which can then be further differentiated into dorsal telencephalic progenitors and glutamatergic neurons efficiently. Overall, our system provides a tool to generate human glutamatergic neurons for researchers to study the development of these neurons and the diseases which affect them.
File Format	PDF HTM / HTML
DOI	10.3791/50321
PubMed reference number	23603787
Journal	Medline
Volume Number	74
Alternate Webpage(s)	https://www.jove.com/pdf-materials/50321/jove-materials-50321-the-specification-telencephalic-glutamatergic-neurons-from-human
Alternate Webpage(s)	https://doi.org/10.3791/50321
Journal	Journal of visualized experiments : JoVE
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article