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Localization of glucokinase-like immunoreactivity in the rat lower brain stem: for possible location of brain glucose-sensing mechanisms.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Maekawa, Fumihiko Toyoda, Yukiyasu Torii, N. Miwa, Ichitomo Thompson, R. Campbell Foster, Douglas L. Tsukahara, Shinji Tsukamura, Hiroko |
| Copyright Year | 2000 |
| Abstract | Pancreatic glucokinase (GK) is considered an important element of the glucose-sensing unit in pancreatic beta-cells. It is possible that the brain uses similar glucose-sensing units, and we employed GK immunohistochemistry and confocal microscopy to examine the anatomical distribution of GK-like immunoreactivities in the rat brain. We found strong GK-like immunoreactivities in the ependymocytes, endothelial cells, and many serotonergic neurons. In the ependymocytes, the GK-like immunoreactivity was located in the cytoplasmic area, but not in the nucleus. The GK-positive ependymocytes were found to have glucose transporter-2 (GLUT2)-like immunoreactivities on the cilia. In addition, the ependymocytes had GLUT1-like immunoreactivity on the cilia and GLUT4-like immunoreactivity densely in the cytoplasmic area and slightly in the plasma membrane. In serotonergic neurons, GK-like immunoreactivity was found in the cytoplasm and their processes. The present results raise the possibility that these GK-like immunopositive cells comprise a part of a vast glucose-sensing mechanism in the brain. |
| File Format | PDF HTM / HTML |
| DOI | 10.1210/en.141.1.375 |
| PubMed reference number | 10614660 |
| Journal | Medline |
| Volume Number | 141 |
| Issue Number | 1 |
| Alternate Webpage(s) | http://endo.endojournals.org/content/141/1/375.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1210/en.141.1.375 |
| Journal | Endocrinology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
