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Nucleoside Diphosphate-sugar 4-Epimerases I. Uridine Diphosphate Glucose 4-Epimerase of Wheat Germ.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Fan, De Fang |
| Copyright Year | 1969 |
| Abstract | Uridine diphosphate (UDP)-glucose 4-epimerase (EC 5.1.3.2) has been purified over 1000-fold from extracts of wheat germ by MnCl(2) treatment, (NH(4))(2)SO(4) fractionation, Sephadex column chromatography, and adsorption onto and elution from calcium phosphate gel. The enzyme has a pH optimum of 9.0. Km values are 0.1 mm for UDP-d-galactose and 0.2 mm for UDP-d-glucose. NAD is required for activity; K(a) = 0.04 mm. NADH is an inhibitor strictly competitive with NAD; K(i) = 2 mum. Wheat germ also contains UDP-l-arabinose 4-epimerase (EC 5.1.3.5) and thymidine diphosphate (TDP)-glucose 4-epimerase which are distinct from UDP-glucose 4-epimerase. |
| Starting Page | 599 |
| Ending Page | 604 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.plantphysiol.org/content/plantphysiol/44/4/599.full.pdf |
| PubMed reference number | 16657106v1 |
| Volume Number | 44 |
| Issue Number | 4 |
| Journal | Plant physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Arabinose Chrysanthemum x morifolium Column Chromatography Galactose Guanosine Diphosphate NADH Nicotinamide adenine dinucleotide (NAD) Nucleoside-Diphosphate Kinase Sugars Thymidine UDP-Glucose 4-Epimerase Uridine Diphosphate Glucose calcium phosphate |
| Content Type | Text |
| Resource Type | Article |
