NDLI: Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein.

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Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein.

Content Provider	Semantic Scholar
Author	Hsieh, Joseph C. Rattner, Amir Smallwood, Philip M. Nathans, Jeremy
Copyright Year	1999
Abstract	Biochemical studies of Wnt signaling have been hampered by difficulties in obtaining large quantities of soluble, biologically active Wnt proteins. In this paper, we report the production in Drosophila S2 cells of biologically active Xenopus Wnt8 (XWnt8). Epitope- or alkaline phosphatase-tagged XWnt8 proteins are secreted by concentrated S2 cells in a form that is suitable for quantitative biochemical experiments with yields of 5 and 0.5 mg per liter, respectively. Conditions also are described for the production in 293 cells of an IgG fusion of the cysteine-rich domain (CRD) of mouse Frizzled 8 with a yield of 20 mg/liter. We demonstrate the use of these proteins for studying the interactions between soluble XWnt8 and various Frizzled proteins, membrane anchored or secreted CRDs, and a set of insertion mutants in the CRD of Drosophila Frizzled 2. In a solid phase binding assay, the affinity of the XWnt8-alkaline phosphatase fusion for the purified mouse Frizzled 8-CRD-IgG fusion is approximately 9 nM.
File Format	PDF HTM / HTML
DOI	10.1073/pnas.96.7.3546
PubMed reference number	10097073
Journal	Medline
Volume Number	96
Issue Number	7
Alternate Webpage(s)	http://www.pnas.org/content/96/7/3546.full.pdf
Alternate Webpage(s)	https://doi.org/10.1073/pnas.96.7.3546
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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