Loading...
Please wait, while we are loading the content...
Similar Documents
Multistage isoelectric focusing in a polymeric microfluidic chip.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cui, Huanchun Horiuchi, Keisuke Dutta, Prashanta Ivory, Cornelius |
| Copyright Year | 2005 |
| Abstract | This paper reports a protocol that improves the resolving power of isoelectric focusing (IEF) in a polymeric microfluidic chip. This method couples several stages of IEF in series by first focusing proteins in a straight channel using broad-range ampholytes and then refocusing segments of the first channel into secondary channels that branch from the first one at T-junctions. Experiments demonstrate that several fluorescent proteins that had focused within a segment of the straight channel in the first stage were refocused at significantly higher resolution due to the shallower pH gradient and higher electrical field gradient. Two variants of green fluorescent protein from the second-stage IEF fractionation were further separated in a third stage. Three stages of IEF were completed in less than 25 min at electric field strengths ranging from 50 to 214 V/cm. |
| Starting Page | 917 |
| Ending Page | 917 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.mstf.wsu.edu/articles/pros_ANA4.pdf |
| PubMed reference number | 16351133v1 |
| Volume Number | 77 |
| Issue Number | 24 |
| Journal | Analytical chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Bands Cascade Device Component Eighty Nine Electroconvulsive Therapy Electrophoresis Electrophoresis, Gel, Pulsed-Field Gradient Green Fluorescent Proteins Isoelectric Focusing Large Microfluidics Organic Chemistry Phenomena Polymers Reagents Solutions Spectrometry Stage level 1 Stage level 3 delta pH pH:LsCnc:Pt:Bld:Qn |
| Content Type | Text |
| Resource Type | Article |
