Effect of thymol on kinetic properties of Ca and K currents in rat skeletal muscle

Content Provider	Semantic Scholar
Author	Szentandrássy, Norbert Szentesi, Péter Nánási, Péter Pál Csernoch, László
Copyright Year	2003
Abstract	BACKGROUND Thymol is widely used as a general antiseptic and antioxidant compound in the medical practice and industry, and also as a stabilizer to several therapeutic agents, including halothane. Thus intoxication with thymol may occur in case of ingestion or improper anesthesia. In the present study, therefore, concentration-dependent effects of thymol (30-600 micro-grams) were studied on calcium and potassium currents in enzymatically isolated rat skeletal muscle fibers using the double vaseline gap voltage clamp technique. RESULTS Thymol suppressed both Ca and K currents in a concentration-dependent manner, the EC50 values were 193 +/- 26 and 93 +/- 11 microM, with Hill coefficients of 2.52 +/- 0.29 and 1.51 +/- 0.18, respectively. Thymol had a biphasic effect on Ca current kinetics: time to peak current and the time constant for inactivation increased at lower (100-200 microM) but decreased below their control values at higher (600 microM) concentrations. Inactivation of K current was also significantly accelerated by thymol (200-300 microM). These effects of thymol developed rapidly and were partially reversible. In spite of the marked effects on the time-dependent properties, thymol caused no change in the current-voltage relationship of Ca and K peak currents. CONCLUSIONS Present results revealed marked suppression of Ca and K currents in skeletal muscle, similar to results obtained previously in cardiac cells. Furthermore, it is possible that part of the suppressive effects of halothane on Ca and K currents, observed experimentally, may be attributed to the concomitant presence of thymol in the superfusate.
Starting Page	9
Ending Page	9
Page Count	1
File Format	PDF HTM / HTML
PubMed reference number	12864924v1
Volume Number	3
Journal	BMC pharmacology
Alternate Webpage(s)	http://download-redirector.springer.com/redirect?contentType=pdf&ddsId=art:10.1186/1471-2210-3-9&originUrl=http://bmcpharma.biomedcentral.com/article/10.1186/1471-2210-3-9
Alternate Webpage(s)	https://dea.lib.unideb.hu/dea/bitstream/handle/2437/110500/file_up_1471-2210-3-9.pdf;jsessionid=31EFE6A971F65C5E7BC3EDF72B2CA4DF?sequence=1
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/f8/ab/1471-2210-3-9.PMC183846.pdf
Language	English
Access Restriction	Open
Subject Keyword	Anti-Infective Agents, Local Calcium Halothane Hippocampus (Brain) Kinetics Muscle Fibers Myalgia Potassium Skeletal Myocytes Skeletal muscle structure Thymol gram
Content Type	Text
Resource Type	Article