NDLI: Umbilical cord blood CD34+ progenitor-derived NK cells efficiently kill ovarian cancer spheroids and intraperitoneal tumors in NOD/SCID/IL2Rgnull mice

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Umbilical cord blood CD34+ progenitor-derived NK cells efficiently kill ovarian cancer spheroids and intraperitoneal tumors in NOD/SCID/IL2Rgnull mice

Content Provider	Semantic Scholar
Author	Evert, Janneke S. Hoogstad-Van Cany, Jeannette Brand, Dirk Van Den Oudenampsen, Manon Brock, Roland Torensma, Ruurd Bekkers, Ruud L. M. Jansen, Joop Massuger, Leon F. A. G. Dolstra, Harry
Copyright Year	2017
Abstract	Adoptive transfer of allogeneic natural killer (NK) cells is an attractive therapy approach against ovarian carcinoma. Here, we evaluated the potency of highly active NK cells derived from human CD34+ haematopoietic stem and progenitor cells (HSPC) to infiltrate and mediate killing of human ovarian cancer spheroids using an in vivo-like model system and mouse xenograft model. These CD56+Perforin+ HSPC-NK cells were generated under stroma-free conditions in the presence of StemRegenin-1, IL-15, and IL-12, and exerted efficient cytolytic activity and IFNγ production toward ovarian cancer monolayer cultures. Live-imaging confocal microscopy demonstrated that these HSPC-NK cells actively migrate, infiltrate, and mediate tumor cell killing in a three-dimensional multicellular ovarian cancer spheroid. Infiltration of up to 30% of total HSPC-NK cells within 8 h resulted in robust tumor spheroid destruction. Furthermore, intraperitoneal HSPC-NK cell infusions in NOD/SCID-IL2Rγnull (NSG) mice bearing ovarian carcinoma significantly reduced tumor progression. These findings demonstrate that highly functional HSPC-NK cells efficiently destruct ovarian carcinoma spheroids in vitro and kill intraperitoneal ovarian tumors in vivo, providing great promise for effective immunotherapy through intraperitoneal HSPC-NK cell adoptive transfer in ovarian carcinoma patients.
File Format	PDF HTM / HTML
DOI	10.1080/2162402X.2017.1320630
Alternate Webpage(s)	https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f6/c8/koni-06-08-1320630.PMC5593716.pdf
PubMed reference number	28919991
Alternate Webpage(s)	https://doi.org/10.1080/2162402X.2017.1320630
Journal	Medline
Volume Number	6
Journal	Oncoimmunology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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