An ultrasensitive electrochemical immunosensor for the detection of human immunoglobulin G based on Ag@BSA microspheres

Content Provider	Semantic Scholar
Author	Zhang, Hongfang Ning, Danlei Zheng, Jianbin
Copyright Year	2015
Abstract	A novel human immunoglobulin G (HIgG) electrochemical immunosensor was developed based on nanosilver-doped bovine serum albumin microspheres (Ag@BSA). The immunosensor was prepared step-wise by first modifying the electrode with β-cyclodextrin functionalized gold nanoparticles followed by the immobilization of captured antibodies and then the formation of a sandwich-type immunocomplex to introduce Ag@BSA bionanoprobes on the sensor surface. The amplification pathway using the stripping voltammetric measurement of silver ions released from Ag@BSA to monitor the immunoreaction was first adopted. The immunosensor exhibited a large dynamic range of 1 fg mL−1 to 10 pg mL−1 and an ultralow detection limit of 0.5 fg mL−1 to HIgG. Moreover, the immunosensor also showed acceptable stability and reproducibility. This biosensor was applied to the detection of the HIgG level in real serum samples.
Starting Page	106607
Ending Page	106612
Page Count	6
File Format	PDF HTM / HTML
DOI	10.1039/C5RA22654F
Volume Number	5
Alternate Webpage(s)	http://www.rsc.org/suppdata/c5/ra/c5ra22654f/c5ra22654f1.pdf
Alternate Webpage(s)	https://doi.org/10.1039/C5RA22654F
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article