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Whole body and hepatic insulin action in normal, starved, and diabetic rats.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Koopmans, Sietse Jan Boer, Sietze F. De Radder, Jasper K. Frölich, Marijke |
| Copyright Year | 1991 |
| Abstract | In normal (N), 3-days starved (S), and streptozotocin-treated (65 mg/kg) 3-days diabetic (D) rats we examined the in vivo dose-response relationship between plasma insulin levels vs. whole body glucose uptake (BGU) and inhibition of hepatic glucose production (HGP) in conscious rats, as determined with the four-step sequential hyperinsulinemic euglycemic clamp technique, combined with [3-3H]glucose infusion. Twelve-hour fasting (basal) HGP was 3.0 +/- 0.2, 2.1 +/- 0.2, and 5.4 +/- 0.5 mg/min in N, S, and D rats, respectively. Next, all rats were clamped at matched glycemia (6 mM). Lowering plasma glucose in D rats from +/- 20 to 6.0 mM did not increase plasma norepinephrine, epinephrine, glucagon, and corticosterone levels. For BGU, insulin sensitivity was increased (70 +/- 11 microU/ml) in S and unchanged (113 +/- 21 microU/ml) in D compared with N rats (105 +/- 10 microU/ml). Insulin responsiveness was unchanged (12.4 +/- 0.8 mg/min) in S and decreased (8.5 +/- 0.8 mg/min) in D compared with N rats (12.3 +/- 0.7 mg/min). For HGP, insulin sensitivity was unchanged (68 +/- 10 microU/ml) in S and decreased (157 +/- 21 microU/ml) in D compared with N rats (71 +/- 5 microU/ml). Insulin responsiveness was identical among N, S, and D rats (complete suppression of HGP). In summary, 1) insulin resistance in D rats is caused by hepatic insensitivity and by a reduction in BGU responsiveness. 2) S rats show normal hepatic insulin action, but insulin sensitivity for BGU is increased. Therefore, S and D rats both suffering from a comparable catabolic state (10-15% body wt loss in 3 days) show opposite effects on in vivo insulin action. This indicates that in vivo insulin resistance in D rats is not caused by the catabolic state per se. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.rug.nl/research/portal/files/3344495/1991AmJPhysiolEMKoopmans.pdf |
| Alternate Webpage(s) | http://www.rug.nl/research/portal/files/3344495/1991AmJPhysiolEMKoopmans.pdf |
| PubMed reference number | 2058659v1 |
| Volume Number | 260 |
| Issue Number | 6 |
| Part | 1 |
| Journal | The American journal of physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Blood Glucose Corticosterone Diabetes Mellitus Epinephrine Glucagon Impaired glucose tolerance Insulin Resistance Liver diseases Milligram per Minute Norepinephrine Streptozocin glucose uptake insulin, isophane |
| Content Type | Text |
| Resource Type | Article |
