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Characterization and sequencing of prototypic human T-lymphotropic virus type 1 (HTLV-1) from an HTLV-1/2 seroindeterminate patient.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Waziri, A. Nagle, James Jacobson, Steve |
| Copyright Year | 2000 |
| Abstract | Serological screening for human T-lymphotropic virus type 1 (HTLV-1) parallels the standard screening process for human immunodeficiency virus (HIV), in which samples found positive by enzyme-linked immunosorbent assay (ELISA) are confirmed with a modified Western blot procedure. There are a significant number of cases in which HTLV-1/2 ELISA-positive specimens demonstrate an incomplete banding pattern on this Western blot. Individuals providing these atypical antibody responses are categorized as seroindeterminate for HTLV-1/2. Although HTLV-1 genomic sequences are readily detectable in the peripheral blood lymphocytes (PBL) of seropositive individuals, previous studies have repeatedly demonstrated that PBL from the vast majority of HTLV-1/2 seroindeterminate individuals are PCR negative for HTLV-1. As a result, identification of the agent responsible for this indeterminate reactivity has been of interest. We have generated an HTLV-1-positive B-cell line (SI-1 B) from one of these seroindeterminate individuals. Previous screening for HTLV-1 in PBL from this patient had been routinely negative by primary PCR; however, HTLV-1 tax had been periodically detected by nested PCR. DNA sequence data generated with genomic DNA from the SI-1 B cell line and HTLV-1-specific primers demonstrated the presence of a full-length viral genome with >97% homology to the Cosmopolitan form of HTLV-1. A 12-bp deletion was identified in the 3'-gag/5'-prot region, which would predict translation of altered or nonfunctional proteins from these genes. We propose that this HTLV-1/2-seroindeterminate patient is infected with a prototypic form of HTLV-1 at an extremely low viral load and that this finding may explain HTLV-1/2 seroindeterminate reactivity in at least a subset of these individuals. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jvi.asm.org/content/74/5/2178.full.pdf |
| PubMed reference number | 10666247v1 |
| Volume Number | 74 |
| Issue Number | 5 |
| Journal | Journal of virology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Biopolymer Sequencing Deletion Mutation Enzyme-Linked Immunosorbent Assay Feline Immunodeficiency Virus HIV Infections Homologous Gene Human T-lymphotropic virus 1 Immunologic Deficiency Syndromes Leukemia, B-Cell Partial Patients Reverse Transcriptase Polymerase Chain Reaction Specimen Subgroup Viral load measurement Western Blot peripheral blood viral capsid secondary envelopment |
| Content Type | Text |
| Resource Type | Article |
