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Assessing enzyme substrate specificity using combinatorial libraries and electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wigger, Maria Nawrocki, Jörg Watson, Celia H. Eyler, John R. Benner, Steven A. |
| Copyright Year | 1997 |
| Abstract | A model experiment for the 'on-line' screening of substrate libraries by enzymes using combinatorial libraries in combination with electrospray ionization-Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry has been performed. The reaction between the electrophilic substrate 1-chloro-2,4-dinitrobenzene and component of a H-gamma-Glu-Cys-Xxx-OH library, catalyzed by glutathione-S-transferase, has been monitored. It shows the feasibility of 'two-dimensional' screening of substrate libraries by ESI-FTICR mass spectrometry. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ffame.org/pubs/Assessing%20enzyme%20substrate%20specificity%20using%20combinatorial%20libraries%20and%20electrospray%20ionization%20Fourier%20transform%20ion%20cyclotron%20resonance%20mass%20spectrometry.pdf |
| PubMed reference number | 9375409 |
| Alternate Webpage(s) | https://doi.org/10.1002/%28SICI%291097-0231%2819971030%2911%3A16%3C1749%3A%3AAID-RCM91%3E3.0.CO%3B2-G |
| Journal | Medline |
| Volume Number | 11 |
| Issue Number | 16 |
| Journal | Rapid communications in mass spectrometry : RCM |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |