NDLI: Identification of Anopheles ( Nyssorhynchus ) marajoara ( Diptera : Culicidae ) in Bolivia Using Polymerase Chain Reaction and a Restriction Endonuclease

Content Provider	Semantic Scholar
Author	Fritz, Gary N. Rodriguez, Rosalba
Copyright Year	2017
Abstract	Anopheles (Nyssorhynchus) marajoara Galvão & Damasceno (Diptera: Culicidae) is a member of the Albitarsis cryptic species complex and is a primary vector of humanPlasmodium in parts of Brazil. We report the Þrst record of An.marajoara in the department of Cochabamba and conÞrm its presence in the department of Santa Cruz, Bolivia. We also describe a PCR protocol producing a single amplicon ( 340 bp) of a section of the internal transcribed spacer 2 speciÞc for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments speciÞc to An. marajoara in Bolivia.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://thekeep.eiu.edu/cgi/viewcontent.cgi?article=1201&context=bio_fac&httpsredir=1&referer=
Alternate Webpage(s)	https://works.bepress.com/gary_fritz/3/download/
Language	English
Access Restriction	Open
Subject Keyword	Culicidae DNA Restriction Enzymes DNA-(apurinic or apyrimidinic site) lyase Diptera Internal transcribed spacer Manihot marajoara Nyssorhynchus Polymerase Chain Reaction Spacer Device Component endonuclease
Content Type	Text
Resource Type	Article

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