NDLI: Comparative proteomic and phosphoproteomic analysis of the silkworm (Bombyx mori) posterior silk gland under high temperature treatment

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Comparative proteomic and phosphoproteomic analysis of the silkworm (Bombyx mori) posterior silk gland under high temperature treatment

Content Provider	Semantic Scholar
Author	Li, Jisheng Ye, Lupeng Lan, Tianyun Yu, Meilan Liang, Jianshe Zhong, Boxiong
Copyright Year	2012
Abstract	The proteins from the posterior silk gland of silkworm hybrids and their parents reared under high temperatures were studied by using comparative proteomic and phosphoproteomic analysis. A total of 82.07, 6.17 and 11.76 % protein spots showed additivity, overdominance and underdominance patterns, respectively. Fifteen differentially expressed protein spots were identified by peptide mass fingerprinting. Among these, four spots, including sHSPs and prohibitin protein that were directly relevant to heat response, were identified. Eleven protein spots were found to play an important role in silk synthesis, and nine protein spots expressed phosphorylation states. According to Gene ontology and KEGG pathway analysis, these nine spots played an important role in stress-induced signal transduction. Expression of most silk synthesis-related proteins was reduced, whereas stress-responsive proteins increased with heat exposure time in three breeds. Furthermore, most proteins showed under- or overdominance in the hybrids compared to the parents. The results suggested that high temperature could alter the expression of proteins related to silk synthesis and heat response in silkworm. Moreover, differentially expressed proteins occurring in the hybrid and its parents may be the main explanation of the observed heterosis.
Starting Page	8447
Ending Page	8456
Page Count	10
File Format	PDF HTM / HTML
DOI	10.1007/s11033-012-1698-5
PubMed reference number	22707192
Journal	Medline
Volume Number	39
Alternate Webpage(s)	http://www.cas.zju.edu.cn/dky2008/userfiles/file/13432057718708.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s11033-012-1698-5
Journal	Molecular Biology Reports
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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