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Colorimetric enumeration of Escherichia coli based on beta-glucuronidase activity.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Adams, Martin R. Grubb, Strangman Hamer, A. Clifford, Michael N. |
| Copyright Year | 1990 |
| Abstract | A medium containing a chromogenic substrate was developed for the enumeration of Escherichia coli on the basis of beta-glucuronidase activity. In this medium there was an inverse linear relationship between the log initial E. coli concentration and the time taken for the color to reach a threshold optical density of 0.05. This relationship applied even when the E. coli population contained 5% beta-glucuronidase-negative cells. Incubation at 44 degrees C reduced the time taken for color development and allowed the procedure to be used in the presence of a competitive microflora that outnumbered the E. coli population by a factor of 10(4). Sodium lauryl sulfate as an additional selective agent gave no significant improvement. In the analysis of environmental water samples, the technique gave a good correlation with a standard cultural method. The procedure shows promise as a simple method for testing the compliance of environmental samples with microbiological criteria for E. coli. |
| File Format | PDF HTM / HTML |
| DOI | 10.1128/aem.56.7.2021-2024.1990 |
| PubMed reference number | 2202252 |
| Journal | Medline |
| Volume Number | 56 |
| Issue Number | 7 |
| Alternate Webpage(s) | http://epubs.surrey.ac.uk/1249/1/fulltext.pdf |
| Alternate Webpage(s) | https://doi.org/10.1128/aem.56.7.2021-2024.1990 |
| Journal | Applied and environmental microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
