Light-dependent assembly of ribulose-1,5-bisphosphate carboxylase.

Content Provider	Semantic Scholar
Author	Bloom, Mark Milos, Patrice M.
Copyright Year	1983
Abstract	Ribulose-1,5-bisphosphate carboxylase [RuP(2)Case; 3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39] is composed of eight small subunits (M(r), 14,000) and eight large subunits (M(r), 55,000). Newly synthesized large subunits are associated with two complexes having sedimentation coefficients of 7 and 29 S. Assembly of RuP(2)Case occurs in isolated intact chloroplasts in the light but not in the dark. When extracts of chloroplasts are treated with ATP or GTP, RuP(2)Case assembly is accelerated while the 29S large subunit complex is maintained. In the presence of Mg(2+), ATP brings about almost complete dissociation of the 29S complex, whereas GTP and a nonhydrolyzable analog of ATP are without effect. These results indicate the existence of a complex set of reactions involving nucleotides, Mg(2+), and several putative intermediates in RuP(2)Case assembly. It is postulated that these reactions at least partly account for the light dependence of RuP(2)Case assembly. In particular, ATP and GTP promote the assembly of large subunits into RuP(2)Case.
File Format	PDF HTM / HTML
DOI	10.1073/pnas.80.4.1013
PubMed reference number	16593277
Journal	Medline
Volume Number	80
Issue Number	4
Alternate Webpage(s)	http://www.pnas.org/content/80/4/1013.full.pdf
Alternate Webpage(s)	https://doi.org/10.1073/pnas.80.4.1013
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article