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Selection for genes encoding secreted proteins and receptors.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Klein, Robert D. Gu, Qimin Goddard, Audrey Rosenthal, André |
| Copyright Year | 1996 |
| Abstract | Extracellular proteins play an essential role in the formation, differentiation, and maintenance of multicellular organisms. Despite that, the systematic identification of genes encoding these proteins has not been possible. We describe here a highly efficient method to isolate genes encoding secreted and membrane-bound proteins by using a single-step selection in yeast. Application of this method, termed signal peptide selection, to various tissues yielded 559 clones that appear to encode known or novel extracellular proteins. These include members of the transforming growth factor and epidermal growth factor protein families, endocrine hormones, tyrosine kinase receptors, serine/threonine kinase receptors, seven transmembrane receptors, cell adhesion molecules, extracellular matrix proteins, plasma proteins, and ion channels. The eventual identification of most, or all, extracellular signaling molecules will advance our understanding of fundamental biological processes and our ability to intervene in disease states. |
| File Format | PDF HTM / HTML |
| DOI | 10.1073/pnas.93.14.7108 |
| PubMed reference number | 8692953 |
| Journal | Medline |
| Volume Number | 93 |
| Issue Number | 14 |
| Alternate Webpage(s) | http://www.pnas.org/content/93/14/7108.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1073/pnas.93.14.7108 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
