Application of specific cell permeable cathepsin G inhibitors resulted in reduced antigen processing in primary dendritic cells.

Content Provider	Semantic Scholar
Author	Reich, Michael Lesner, Adam Lęgowska, Anna Sieńczyk, Marcin Oleksyszyn, Józef Boehm, Bernhard Otto Burster, Timo
Copyright Year	2009
Abstract	The serine protease cathepsin G (CatG) is expressed in primary antigen-presenting cells and regulates autoantigen processing in CatG pre-loaded fibroblasts. To further investigate the function of CatG in the major histocompatibility complex (MHC) class II loading compartments, a specific, cell permeable CatG-inhibitor is needed. In this study, several CatG-inhibitors were tested for their ability to penetrate the cell membrane of peripheral blood mononuclear cells (PBMC). We find that the commercially available reversible CatG-specific inhibitor I (CatG inhibitor) and the irreversible Suc-Val-Pro-Phe(P) (OPh)(2) (Suc-VPF) are both cell permeable and specifically inhibit intracellular CatG in the PBMC. Furthermore, selective inhibition of CatG resulted in reduced tetanus toxin C-fragment (TTC) and hemagglutinin (HA) processing and presentation to CD4(+) T cells. We conclude that these CatG inhibitors can be used for both antigen-processing studies and for modulation of T cell response in situ and in vivo.
File Format	PDF HTM / HTML
DOI	10.1016/j.molimm.2009.06.017
PubMed reference number	19615749
Journal	Medline
Volume Number	46
Issue Number	15
Alternate Webpage(s)	http://www.biomedproofreading.com/cathepsin.pdf
Alternate Webpage(s)	https://doi.org/10.1016/j.molimm.2009.06.017
Journal	Molecular immunology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article