Differential partitioning of pulmonary surfactant protein SP-A into regions of monolayers of dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylcholine/dipalmitoylphosphatidylglycerol.

Content Provider	Semantic Scholar
Author	Ruano, M. Lobo Nag, Khiromani Worthman, L. A. Casals, Cristina Pérez-Gil, Jesús Keough, Kevin M. W.
Copyright Year	1998
Abstract	The interaction of the pulmonary surfactant protein SP-A fluorescently labeled with Texas Red (TR-SP-A) with monolayers of dipalmitoylphosphatidylcholine (DPPC) and DPPC/dipalmitoylphosphatidylglycerol 7:3 w/w has been investigated. The monolayers were spread on aqueous subphases containing TR-SP-A. TR-SP-A interacted with the monolayers of DPPC to accumulate at the boundary regions between liquid condensed (LC) and liquid expanded (LE) phases. Some TR-SP-A appeared in the LE phase but not in the LC phase. At intermediate surface pressures (10-20 mN/m), the protein caused the occurrence of more, smaller condensed domains, and it appeared to be excluded from the monolayers at surface pressure in the range of 30-40 mN/m. TR-SP-A interaction with DPPC/dipalmitoylphosphatidylglycerol monolayers was different. The protein did not appear in either LE or LC but only in large aggregates at the LC-LE boundary regions, a distribution visually similar to that of fluorescently labeled concanavalin A adsorbed onto monolayers of DPPC. The observations are consistent with a selectivity of interaction of SP-A with DPPC and for its accumulation in boundaries between LC and LE phase.
File Format	PDF HTM / HTML
DOI	10.1016/S0006-3495(98)77828-2
Alternate Webpage(s)	https://core.ac.uk/download/pdf/82572024.pdf
Alternate Webpage(s)	http://pendientedemigracion.ucm.es/info/respira/resources/PDF/047.pdf
PubMed reference number	9512012
Alternate Webpage(s)	https://doi.org/10.1016/S0006-3495%2898%2977828-2
Journal	Medline
Volume Number	74
Issue Number	3
Journal	Biophysical journal
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article