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Simultaneous Determination of Six Phenolic Constituents of Danshen Injection in Rat Plasma by Liquid Chromatography—Electrospray Ionization—Mass Spectrometry and its Application to a Pharmacokinetic Study
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhao, Di Han, De-En Li, Ning Lu, Yang Li, Ting-Ting Yang, Sun Hee He, Jia-Ke Chen, Xi-Jing |
| Copyright Year | 2011 |
| Abstract | Salvianolic acid A, salvianolic acid B, danshensu, protocatechuic aldehyde, rosmarinic acid and lithospermic acid are the six major active constituents in Danshen injection. In this study, a rapid, sensitive and specific liquid chromatographic–electrospray ionization–mass spectrometry method for the simultaneous quantitative determination of these compounds in rat plasma was developed. After a single step of liquid–liquid extraction with ethyl acetate, they were eluted by a Hypersil C18 column (5 μm, i.d. 4.6 × 200 mm) within 4 min with a mobile phase consisting of acetonitrile and 0.1% formic acid water solution (35:65, v/v). The assay was linear in the concentration range of 0.05–10 μg mL−1. Absolute recoveries were above 60%. The precisions and accuracies determined within three consecutive days were within acceptable limits. The method was successfully applied to a pharmacokinetic study in rats after an intravenous administration of Danshen injection. |
| Starting Page | 395 |
| Ending Page | 403 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1255/ejms.1137 |
| PubMed reference number | 22006631 |
| Journal | Medline |
| Volume Number | 17 |
| Alternate Webpage(s) | http://www.dmpkd.com/doc/2011paper08.pdf |
| Alternate Webpage(s) | https://doi.org/10.1255/ejms.1137 |
| Journal | European journal of mass spectrometry |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
