Loading...
Please wait, while we are loading the content...
Similar Documents
The RNA-dependent RNA polymerase, QDE-1, is a rate-limiting factor in post-transcriptional gene silencing in Neurospora crassa.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Forrest, Emma C. Cogoni, Carlo Macino, Giuseppe |
| Copyright Year | 2004 |
| Abstract | The RNA-dependent RNA polymerase (RdRP) qde-1 is an essential component of post-transcriptional gene silencing (PTGS), termed 'quelling' in the fungus Neurospora crassa. Here we show that the overexpression of QDE-1 results in a dramatic increase in the efficiency of quelling, with a concomitant net increase in the quantity of al-1 siRNAs. Moreover, in overexpressed strains there is a significant reduction in the number of transgenes required to induce quelling, and an increase in the phenotypic stability despite progressive loss of tandemly repeated transgenes, which normally determines reversion of a silenced phenotype to wild type. These data suggest that the activation and maintenance of silencing in Neurospora appear to rely both on the cellular amount of QDE-1 and the amount of transgenic copies producing RNA molecules that act as a substrate for the RdRP, implicating QDE-1 as a rate-limiting factor in PTGS. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://nar.oxfordjournals.org/content/32/7/2123.full.pdf |
| PubMed reference number | 15090622v1 |
| Volume Number | 32 |
| Issue Number | 7 |
| Journal | Nucleic acids research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Copy (object) DNA-Directed RNA Polymerase Ephrin Type-B Receptor 1, human Fungi Neurospora sitophila Ab.IgE:ACnc:Pt:Ser:Qn RNA Interference RNA-directed RNA polymerase activity Transcription, Genetic Transgenes Wild Type |
| Content Type | Text |
| Resource Type | Article |
