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Tumor necrosis factor-alpha in ischemia and reperfusion injury in rat lungs.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Khimenko, Pavel L. Bagby, Gregory J. Fuseler, John W. Taylor, Aubrey E. |
| Copyright Year | 1998 |
| Abstract | The effects of both recombinant rat tumor necrosis factor-alpha (TNF-alpha) and an anti-TNF-alpha antibody were studied in isolated buffer-perfused rat lungs subjected to either 45 min of nonventilated [ischemia-reperfusion (I/R)] or air-ventilated (V/R) ischemia followed by 90 min of reperfusion and ventilation. In the I/R group, the vascular permeability, as measured by the filtration coefficient (Kfc), increased three- and fivefold above baseline after 30 and 90 min of reperfusion, respectively (P < 0.001). Over the same time intervals, the Kfc for the V/R group increased five- and tenfold above baseline values, respectively (P < 0.001). TNF-alpha measured in the perfusates of both ischemic models significantly increased after 30 min of reperfusion. Recombinant rat TNF-alpha (50,000 U), placed into perfusate after baseline measurements, produced no measurable change in microvascular permeability in control lungs perfused over the same time period (135 min), but I/R injury was significantly enhanced in the presence of TNF-alpha. An anti-TNF-alpha antibody (10 mg/rat) injected intraperitoneally into rats 2 h before the lung was isolated prevented the microvascular damage in lungs exposed to both I/R and V/R (P < 0.001). These results indicate that TNF-alpha is an essential component at the cascade of events that cause lung endothelial injury in short-term I/R and V/R models of lung ischemia. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 9843519 |
| Journal | Medline |
| Volume Number | 85 |
| Issue Number | 6 |
| Alternate Webpage(s) | http://jap.physiology.org/content/jap/85/6/2005.full.pdf |
| Journal | Journal of applied physiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
