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Adhesion of Pseudomonas fluorescens biofilms to glass, stainless steel and cellulose
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dagang, Wan Rosmiza Zana Wan Bowen, James O'Keeffe, Johnny Robbins, Phillip T. Zhang, Zhuofu |
| Copyright Year | 2016 |
| Abstract | ObjectivesThe adhesion of colloidal probes of stainless steel, glass and cellulose to Pseudomonas fluorescens biofilms was examined using atomic force microscopy (AFM) to allow comparisons between surfaces to which biofilms might adhere.ResultsBiofilm was grown on a stainless steel substrate and covered most of the surface after 96 h. AFM approach and retraction curves were obtained when the biofilm was immersed in a tryptone/soy medium. On approach, all the colloidal probes experienced a long non-contact phase more than 100 nm in length, possibly due to the steric repulsion by extracellular polymers from the biofilm and hydrophobic effects. Retraction data showed that the adhesion varied from position to position on the biofilm. The mean value of adhesion of glass to the biofilm (48 ± 7 nN) was the greatest, followed by stainless steel (30 ± 7 nN) and cellulose (7.8 ± 0.4 nN).ConclusionThe method allows understanding of adhesion between the three materials and biofilm, and development of a better strategy to remove the biofilm from these surfaces relevant to different industrial applications. |
| Starting Page | 787 |
| Ending Page | 792 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s10529-016-2047-x |
| Alternate Webpage(s) | http://oro.open.ac.uk/45977/1/%5B63%5D%20Adhesion%20of%20Pseudomonas%20fluorescens%20biofilms.pdf |
| Alternate Webpage(s) | https://research.birmingham.ac.uk/portal/files/25247160/Wan_Dagang_et_al_Biotechnology_Letters_accepted.pdf |
| PubMed reference number | 26892223 |
| Alternate Webpage(s) | https://doi.org/10.1007/s10529-016-2047-x |
| Journal | Medline |
| Volume Number | 38 |
| Journal | Biotechnology Letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
