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Aislamiento y caracterización morfológica de cepas nativas de actinomicetos y su actividad antagónica contra Ralstonia solanacearum, Escherichia coli, Staphylococcus aureus y Salmonella sp.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Guevara, Luis Ever Trujillo Bryan, Lowell L. |
| Copyright Year | 2017 |
| Abstract | Among the great variety of soil-bacteria are actinomycetes, which have been extensively studied because of their antimicrobial potential of medical and commercial interest. The objective of this study was to isolate, purify and describe the characteristics of colonial and cellular morphology and to identify the possible genera of actinomycetes strains obtained from soil samples from four different sites in the Uyuca Biological Reserve (RBU) located in the department of Francisco Morazán, Honduras. A total of 12 strains were isolated and subjected to biochemical tests such as Gram staining, oxidase, catalase, and morphological comparison using the Bergey manual, which identified eight strains of possible genus Nocardia, two of Streptomyces and two of Terrabacter. The antagonistic activity of each strain was evaluated against four pathogenic bacteria: Ralstonia solanacearum; Escherichia coli, Staphylococcus aureus and Salmonella sp. Five strains were found to have inhibitory properties against Staphylococcus aureus, two strains against Ralstonia solanacearum, one strain against Escherichia coli and one strain against Salmonella sp. A collection was established by inoculation of the strains on sterile soil and inclined oat agar. It was concluded that the soil of the RBU contains bacteria of the class Actinobacteria with potential inhibitory characteristics against these four pathogenic bacteria. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://bdigital.zamorano.edu/bitstream/11036/5968/1/IAD-2017-015.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |