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Protein kinase C regulates chondrogenesis of mesenchymes via mitogen-activated protein kinase signaling.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chang, Sandra H. Yang, M. S. Kang, Sang Sun Sonn, Jong Kyung Chun, Jang Soo |
| Copyright Year | 1998 |
| Abstract | A possible regulatory mechanism of protein kinase C (PKC) in the chondrogenesis of chick limb bud mesenchymes has been investigated. Inhibition or down-regulation of PKC resulted in the activation of a mitogen-activated protein kinase subtype Erk-1 and the inhibition of chondrogenesis. On the other hand, inhibition of Erk-1 with PD98059 enhanced chondrogenesis and relieved PKC-induced blockage of chondrogenesis. Erk-1 inhibition, however, did not affect expression and subcellular distribution of PKC isoforms expressed in mesenchymes nor cell proliferation. The results suggest that PKC regulates chondrogenesis by modulating Erk-1 activity. Inhibition or depletion of PKC inhibited proliferation of chondrogenic competent cells, and Erk-1 inhibition did not affect PKC modulation of cell proliferation. However, PKC-induced modulation of expression of cell adhesion molecules involved in precartilage condensation was reversed by the inhibition of Erk-1. Expression of N-cadherin was detected at the early period of chondrogenesis. Inhibition or depletion of PKC induced sustained expression of N-cadherin, and Erk-1 inhibition blocked the effects of PKC modulation. The expression of integrin alpha5 beta1 and fibronectin was found to be increased transiently during chondrogenesis. Depletion or inhibition of PKC caused a continuous increase of the expression of these molecules throughout the culture period, and Erk-1 inhibition abolished the modulating effects of PKC. Because reduction of the examined cell adhesion molecule expression is a prerequisite for the progression of chondrogenesis after cell condensation, our results indicate that PKC regulates chondrogenesis by modulating expression of these molecules via Erk-1 signaling. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jbc.org/content/273/30/19213.full.pdf |
| PubMed reference number | 9668109v1 |
| Volume Number | 273 |
| Issue Number | 30 |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Bud - CHV concept Cadherin-2 Cadherins Cell Adhesion Molecules Cell Proliferation Chondrogenesis Down-Regulation Limb Buds Mitogen-Activated Protein Kinases Mitogens PD 98059 Protein Isoforms Protein Kinase C |
| Content Type | Text |
| Resource Type | Article |
