Probing DNA-DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics.

Content Provider	Semantic Scholar
Author	Brouwer, Ineke King, Graeme A. Heller, Iddo Biebricher, Andreas S. Peterman, Erwin J. G. Wuite, Gijs J. L.
Copyright Year	2017
Abstract	DNA metabolism and DNA compaction in vivo involve frequent interactions of remote DNA segments, mediated by proteins. In order to gain insight into such interactions, quadruple-trap optical tweezers have been developed. This technique provides an unprecedented degree of control through the ability to independently manipulate two DNA molecules in three dimensions. In this way, discrete regions of different DNA molecules can be brought into contact with one another, with a well-defined spatial configuration. At the same time, the tension and extension of the DNA molecules can be monitored. Furthermore, combining quadruple-trap optical tweezers with microfluidics makes fast buffer exchange possible, which is important for in situ generation of the dual DNA-protein constructs needed for these kinds of experiments. In this way, processes such as protein-mediated inter-DNA bridging can be studied with unprecedented control. This chapter provides a step-by-step description of how to perform a dual DNA manipulation experiment using combined quadruple-trap optical tweezers and microfluidics.
Starting Page	275
Ending Page	293
Page Count	19
File Format	PDF HTM / HTML
DOI	10.1007/978-1-4939-6421-5_10
PubMed reference number	27844432
Journal	Medline
Volume Number	1486
Alternate Webpage(s)	http://dare.ubvu.vu.nl/bitstream/handle/1871/54585/chapter%202.pdf?isAllowed=y&sequence=7
Alternate Webpage(s)	https://doi.org/10.1007/978-1-4939-6421-5_10
Journal	Methods in molecular biology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article