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Functional analysis of the superoxide dismutase family in Aspergillus fumigatus.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lambou, Karine Lamarre, Claude Beau, Rémi Dufour, Nicolas Latgé, J. -P. |
| Copyright Year | 2010 |
| Abstract | Reactive oxidant species produced by phagocytes have been reported as being involved in the killing of Aspergillus fumigatus. Fungal superoxide dismutases (SODs) that detoxify superoxide anions could be putative virulence factors for this opportunistic pathogen. Four genes encoding putative Sods have been identified in the A. fumigatus genome: a cytoplasmic Cu/ZnSOD (AfSod1p), a mitochondrial MnSOD (AfSod2p), a cytoplasmic MnSOD (AfSod3p) and AfSod4 displaying a MnSOD C-terminal domain. During growth, AfSOD1 and AfSOD2 were highly expressed in conidia whereas AfSOD3 was only strongly expressed in mycelium. AfSOD4 was weakly expressed compared with other SODs. The deletion of AfSOD4 was lethal. Delta sod1 and Delta sod2 mutants showed a growth inhibition at high temperature and a hypersensitivity to menadione whereas the sod3 mutant had only a slight growth delay at high temperature. Multiple mutations had only an additive effect on the phenotype. The triple sod1/sod2/sod3 mutant was characterized by a delay in conidial germination, a reduced conidial survival during storage overtime, the highest sensitivity to menadione and an increased sensitivity to killing by alveolar macrophage of immunocompetent mice. In spite of these phenotypes, no significant virulence difference was observed between the triple mutant and parental strain in experimental murine aspergillosis models in immunocompromised animals. |
| Starting Page | 2249 |
| Ending Page | 2263 |
| Page Count | 15 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bichat.inserm.fr/equipes/emi0339/publications_pdf/exhaustiveliste/SODetAspergillusMolMicrob.pdf |
| PubMed reference number | 20487287v1 |
| Alternate Webpage(s) | https://doi.org/10.1111/j.1365-2958.2009.07024.x |
| DOI | 10.1111/j.1365-2958.2009.07024.x |
| Journal | Molecular microbiology |
| Volume Number | 75 |
| Issue Number | 4 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Anions Aspergillosis Aspergillus fumigatus Deletion Mutation Delta aminolevulinate:SCnc:Pt:Urine:Qn Drug Allergy Fever Germination Growth delay Immunocompetent Macrophages, Alveolar Mycelium Oxidants Pathogenic organism Phagocytes Phenotype SOD2 protein, human Superoxides Virulence Vitamin K 3 gamma-delta T-Cell Receptor mutant superoxide dismutase activity |
| Content Type | Text |
| Resource Type | Article |
