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Rapid and sensitive static headspace gas chromatography-mass spectrometry method for the analysis of ethanol and abused inhalants in blood.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wasfi, Ibrahim A. Al-Awadhi, Ahmed Hassan Al-Hatali, Zainat Naser Al-Rayami, Fatima Juma Al katheeri, Nawal A. |
| Copyright Year | 2004 |
| Abstract | A sensitive and specific method using static headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in biological fluids using n-propanol as internal standard. Gas chromatography was performed in isothermal mode with a GC run time of 2.6 min. The quantification was performed using scan mode abstracting a quantitative ion and a qualifier ion for ethanol and for the internal standard. The method was linear (r(2), 0.999, in the concentration range of 5-200 mg/dl), specific (no interference from methanol acetaldehyde, acetone or from endogenous materials), sensitive (limit of quantification and limit of detection of 0.2 and 0.02 mg/dl, respectively) and robust (less than 5% inter- and intra-assay coefficient of variation). A slightly modified method was also developed for the quantification of five commonly abused inhalants (dichloromethane, ethyl acetate, benzene, toluene and xylene) in blood. The method used a gradient GC program with a run time of 8 min. The quantification was performed using scan mode and integrating the area under the peak using trichloroethane as an internal standard. Without optimization, the method was linear (from 5 to 100 mg/l) and sensitive. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://quimica.udea.edu.co/~forense/etoh_blod_2004.pdf |
| PubMed reference number | 14670752v1 |
| Volume Number | 799 |
| Issue Number | 2 |
| Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 1,1,1-trichloroethane 1-Propanol Abstract Summary Acetaldehyde Acetone Coefficient Ethanol Gas Chromatography Gas Chromatography-Mass Spectrometry Gases Ions Liquid substance Methanol Microgram per Milliliter Quantitation Toluene Xylene ethyl acetate methanol-CoM methyltransferase complex methylene chloride mg/dl |
| Content Type | Text |
| Resource Type | Article |
