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Enzyme-linked immunosorbent assay for Helicobacter pylori needs adjustment for the population investigated.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hoang, Thi Thu Ha Wheeldon, Thuc-Uyen Bengtsson, Carina Phung, Dac Cam Sörberg, Mikael Granström, Marta |
| Copyright Year | 2004 |
| Abstract | Helicobacter pylori infection and peptic ulcer disease are common in developing countries, e.g., Vietnam. An enzyme-linked immunosorbent assay (ELISA) for screening of patients and for seroepidemiology is a useful tool but needs to be validated in the population studied. We used in-house ELISA with sonicated Swedish and Vietnamese strains as antigens to measure immunoglobulin G antibodies after absorption with sonicated Campylobacter jejuni in sera from 270 H. pylori culture-confirmed peptic ulcer patients, 128 Swedish urea-breath test and immunoblot-positive healthy controls, and 432 Vietnamese immunoblot-positive population controls. Sonicated whole-cell antigen based on the local strains showed a significantly better performance. Immunoblot-positive peptic ulcer patients had significantly higher antibody concentrations than immunoblot-positive population controls, necessitating a lower cutoff level if serology is used for screening or epidemiological purposes. The study shows that the parameters of ELISA for H. pylori need to be adjusted for the population being investigated. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jcm.asm.org/content/42/2/627.full.pdf |
| PubMed reference number | 14766827v1 |
| Volume Number | 42 |
| Issue Number | 2 |
| Journal | Journal of clinical microbiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Breath Tests Campylobacter jejuni Enzyme-Linked Immunosorbent Assay Epidemiology Helicobacter pylori infection Immunoblotting Patients Peptic Ulcer Urea |
| Content Type | Text |
| Resource Type | Article |
