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Carbachol-induced Suppression of Contraction Rhythm in Spontaneously Beating Cultured Cardiac Myocytes From Neonatal Rats
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kohashi, Masayuki Kawahara, Ko-Ichi Yamauchi, Yoshiko |
| Copyright Year | 2004 |
| Abstract | Nitric oxide (NO) and reactive oxygen species (ROS) are known to play various functional and pathophysiological roles as an intracellular messenger in the heart. In this study, we investigated whether the increased production of NO and/or ROS was involved in the cholinergic regulation of rhythmic contraction in spontaneously beating cultured cardiac myocytes from neonatal rats. Exposure of cultures to carbachol, an agonist of muscarinic acetylcholine receptors (mAchR), produced a dose-dependent decrease in the beat rate of cultured cardiac myocytes, and such a effect was significantly attenuated by pre-treatment with an NOS inhibitor, as well as an NO scavenger. In addition, exposure to an NO donor (SNAP) also decreased the beat rate dose-dependently. Carbachol- or SNAP-induced suppression of the contraction rhythm was significantly attenuated by co-treatment with 5-hydroxydecanoate (5-HD). In contrast, treatment with diazoxide decreased the beat rate dose-dependently. Carbachol treatment increased the in... |
| Starting Page | 367 |
| Ending Page | 381 |
| Page Count | 15 |
| File Format | PDF HTM / HTML |
| DOI | 10.1076/brhm.34.4.367.26224 |
| Volume Number | 34 |
| Alternate Webpage(s) | https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/5934/1/BRR34-4.pdf |
| Alternate Webpage(s) | https://doi.org/10.1076/brhm.34.4.367.26224 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
