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Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probes.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dennis, Allison M. Bao, Gang |
| Copyright Year | 2008 |
| Abstract | Fluorescence resonance energy transfer (FRET) characteristics, including the efficiency, donor-acceptor distance, and binding strength of six fluorescent protein (FP)-quantum dot (QD) pairs were quantified, demonstrating that FPs are efficient acceptors for QD donors with up to 90% quenching of QD fluorescence and that polyhistidine coordination to QD core-shell surface is a straightforward and effective means of conjugating proteins to commercially available QDs. This provides a novel approach to developing QD-based FRET probes for biomedical applications. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 18412403 |
| Journal | Medline |
| Volume Number | 8 |
| Issue Number | 5 |
| Alternate Webpage(s) | http://sites.bu.edu/dennislab/files/2014/08/2008-Nano-Letters.pdf |
| Alternate Webpage(s) | https://doi.org/10.1021/nl080358%2B |
| Journal | Nano letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |