Loading...
Please wait, while we are loading the content...
Similar Documents
Coccidioidomycosis and blastomycosis: advances in molecular diagnosis.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bialek, Ralf González, Gloria María Begerow, Dominik Zelck, Ulrike E. |
| Copyright Year | 2005 |
| Abstract | Clinical isolates of Coccidioides spp. and Blastomyces dermatitidis can be identified by chemiluminescent DNA probes and PCR assays targeting multicopy genes. In fixed tissue samples, cells of the two fungi are specified by in situ hybridization and PCR assays targeting 18S rDNA but sequencing of the products is mandatory. Nested PCR assays targeting genes encoding species- or genus-specific proteins like proline rich antigen of Coccidioides spp. and B. dermatitidis adhesin facilitate amplification of specific DNA from fixed tissue samples. The value of DNA amplification from native specimens of suspected cases of coccidioidomycosis or blastomycosis still needs to be determined. |
| Starting Page | 45 |
| Ending Page | 50 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ruhr-uni-bochum.de/imperia/md/content/geobot/2005/bialek_2005-coccidioidomycosis.pdf |
| PubMed reference number | 16043334v1 |
| Volume Number | 45 |
| Issue Number | 3 |
| Journal | FEMS immunology and medical microbiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adhesins, Bacterial Biopolymer Sequencing Blastomyces dermatitidis Blastomycosis Coccidioides Coccidioidomycosis DNA Probes Genus In Situ Hybridization Mandatory - HL7DefinedRoseProperty Nucleic Acid Hybridization Proline Reverse Transcriptase Polymerase Chain Reaction Specimen cellular targeting dna amplification |
| Content Type | Text |
| Resource Type | Article |
