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Identification of the Actinobacillus pleuropneumoniae leucine-responsive regulatory protein and its involvement in the regulation of in vivo-induced genes.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wagner, Trevor K. |
| Copyright Year | 2007 |
| Abstract | Actinobacillus pleuropneumoniae is a gram-negative bacterial pathogen that causes a severe hemorrhagic pneumonia in swine. We have previously shown that the limitation of branched-chain amino acids (BCAAs) is a cue that induces the expression of a subset of A. pleuropneumoniae genes identified as specifically induced during infection of the natural host animal by using an in vivo expression technology screen. Leucine-responsive regulatory protein (Lrp) is a global regulator and has been shown in Escherichia coli to regulate many genes, including genes involved in BCAA biosynthesis. We hypothesized that A. pleuropneumoniae contains a regulator similar to Lrp and that this protein is involved in the regulation of a subset of genes important during infection and recently shown to have increased expression in the absence of BCAAs. We report the identification of an A. pleuropneumoniae serotype 1 gene encoding a protein with similarity to amino acid sequence and functional domains of other reported Lrp proteins. We further show that purified A. pleuropneumoniae His6-Lrp binds in vitro to the A. pleuropneumoniae promoter regions for ilvI, antisense cps1AB, lrp, and nqr. A genetically defined A. pleuropneumoniae lrp mutant was constructed using an allelic replacement and sucrose counterselection method. Analysis of expression from the ilvI and antisense cps1AB promoters in wild-type, lrp mutant, and complemented lrp mutant strains indicated that Lrp is required for induction of expression of ilvI under BCAA limitation. |
| Starting Page | 91 |
| Ending Page | 103 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://iai.asm.org/content/75/1/91.full.pdf |
| PubMed reference number | 17060463v1 |
| Volume Number | 75 |
| Issue Number | 1 |
| Journal | Infection and immunity |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Actinobacillus pleuropneumoniae Amino Acid Sequence Amino Acids Amino Acids, Branched-Chain Anabolism Leucine Pathogenic organism Promoter Regions, Genetic Staphylococcal Protein A Subgroup Sucrose Sus scrofa antisense therapy gram haemorrhagic pneumonia regulatory proteins |
| Content Type | Text |
| Resource Type | Article |
