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Mind the gaps: confocal endomicroscopy showed increased density of small bowel epithelial gaps in inflammatory bowel disease.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liu, Julia J. Madsen, Karen L. Boulanger, Pierre A. Dieleman, Levinus Albert Meddings, Jonathan Ian Fedorak, Richard N. |
| Copyright Year | 2011 |
| Abstract | OBJECTIVES Confocal endomicroscopy can be used to image intestinal mucosa. Epithelial gaps resulting from shedding of epithelial cells have been reported in patients. We hypothesize that the rate of epithelial cell shedding increases in patients with Crohn's disease, leading to more epithelial gaps and barrier dysfunction. In this study, we used probe-based confocal laser endomicroscopy to quantify epithelial cells and gaps in patients with Crohn's disease compared with controls. We also determined the density of epithelial gaps in a mouse model of inflammatory bowel disease-interleukin-10-deficient (IL-10) mice, versus the background strain using rigid probe confocal endomicroscopy. METHODS Probe-based confocal laser endomicroscopy of the terminal ileum of both patients with Crohn's disease and controls was performed by a single endoscopist during colonoscopy. In mice, sections of the small intestine were imaged using a rigid confocal probe. Gap density was defined as the number of epithelial gaps per 1000 cells counted. RESULTS In this study, we examined 6 controls (2 male and 4 female; median age 59 y) and 8 patients with Crohn's disease (5 male and 3 female; median age 42 y). The mean gap densities (±standard error) observed for the 2 groups were 17.7±5.6 and 117±33 gaps per 1000 cells, respectively (P<0.01). For control and IL-10 mice, the gap densities were 10.5±2.2 and 17.8±1.4 gaps per 1000 cells, respectively (P<0.01). CONCLUSIONS The epithelial gap density was significantly higher in patients with Crohn's disease than controls. Gap density was also elevated in the mouse model of inflammatory bowel disease. |
| Starting Page | 1 |
| Ending Page | 7 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://webdocs.cs.ualberta.ca/~wfb/ammi/publications/JCG-2010.pdf |
| PubMed reference number | 21030873v1 |
| Alternate Webpage(s) | https://doi.org/10.1097/MCG.0b013e3181fbdb8a |
| DOI | 10.1097/MCG.0b013e3181fbdb8a |
| Journal | Journal of clinical gastroenterology |
| Volume Number | 45 |
| Issue Number | 3 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Confocal Laser Endomicroscopy Crohn Disease Distal part of ileum Epithelial Cells Fifty Nine Inflammatory Bowel Diseases Intestinal Diseases Intestinal Mucosa Intestines Intestines, Small Muscle Rigidity One Thousand Paget's Disease, Mammary Patients Small Intestinal Wall Tissue density |
| Content Type | Text |
| Resource Type | Article |
