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One-tube post-PCR fluorescent labeling of DNA fragments.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Inazuka, M. Tahira, Tomoko Hayashi, Kenshi |
| Copyright Year | 1996 |
| Abstract | A method for fluorescent postlabeling of PCR products has been developed. The method uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR-amplified DNA fragment for fluorescent nucleotides. All reactions, including PCR, are performed in one tube simply by successive addition of reagents. The products can be applied directly to fluorescence-based automated DNA sequencers without purification for either length determination in denaturing electrophoresis or mutation detection in SSCP electrophoresis. |
| Starting Page | 19 |
| Ending Page | 31 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://genome.cshlp.org/content/6/6/551.full.pdf |
| PubMed reference number | 8828044v1 |
| Volume Number | 6 |
| Issue Number | 6 |
| Journal | Genome research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Electrophoresis Fluorescence Nucleotides Reagents efmoroctocog alfa 1 UNT Injection [Eloctate] |
| Content Type | Text |
| Resource Type | Article |
