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Human Cytochrome P450 (CYP1A2)‐dsDNA Interaction In Situ Evaluation using a dsDNA‐Electrochemical Biosensor
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lopes, Ilanna Campelo Oliveira-Brett, Ana Maria |
| Copyright Year | 2017 |
| Abstract | Human cytochrome CYP1A2 is one of the major hepatic cytochrome P450s involved in many drugs metabolism, and chemical carcinogens activation. The CYP1A2-dsDNA interaction in situ evaluation using a DNA-electrochemical biosensor and differential pulse voltammetry was investigated. A dsDNA-electrochemical biosensor showed that CYP1A2 interacted with dsDNA causing conformational changes in the double helix chain and DNA oxidative damage. A preferential interaction between the dsDNA guanosine residues and CYP1A2 was found, as free guanine and 8-oxoguanine, a DNA oxidative damage biomarker, oxidation peaks were detected. This was confirmed using guanine and adenine homopolynucleotides-electrochemical biosensors. The CYP1A2-dsDNA interaction and dsDNA conformation changes was also confirmed by UV-Vis spectrophotometry. |
| Starting Page | 1674 |
| Ending Page | 1682 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.1002/elan.201600713 |
| Alternate Webpage(s) | http://www1.ci.uc.pt/pessoal/anabrett/pdfs/PDF-2017/217-Electroanalysis-2017.pdf |
| Alternate Webpage(s) | https://doi.org/10.1002/elan.201600713 |
| Volume Number | 29 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |