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Osmoregulated periplasmic glucans of Salmonella enterica serovar Typhimurium are required for optimal virulence in mice.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bhagwat, Arvind A. Jun, Won Kyung Liu, Liu Kannan, Porteen Dharne, Mahesh S. Pheh, Benedict Tall, Ben D. Kothary, Mahendra H. Gross, Kenneth. Angle, Siddhesh R. Meng, Jianghong Smith, Allen |
| Copyright Year | 2009 |
| Abstract | We purified osmoregulated periplasmic glucans (OPGs) from Salmonella enterica serovar Typhimurium and found them to be composed of 100 % glucose with 2-linked glucose as the most abundant residue, with terminal glucose, 2,3-linked and 2,6-linked glucose also present in high quantities. The two structural genes for OPG biosynthesis, opgG and opgH, form a bicistronic operon, and insertion of a kanamycin resistance gene cassette into this operon resulted in a strain devoid of OPGs. The opgGH mutant strain was impaired in motility and growth under low osmolarity conditions. The opgGH mutation also resulted in a 2 log increase in the LD50 in mice compared to the wild-type strain SL1344. Inability to synthesize OPGs had no significant impact on the organism's lipopolysaccharide pattern or its ability to survive antimicrobial peptides-, detergent-, pH- and nutrient-stress conditions. We observed that the opgGH-defective strain respired at a reduced rate under acidic growth conditions (pH 5.0) and had lower ATP levels compared to the wild-type strain. These data indicate that OPGs of S. Typhimurium contribute towards mouse virulence as well as growth and motility under low osmolarity growth conditions. |
| Starting Page | 590 |
| Ending Page | 595 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://naldc.nal.usda.gov/download/43675/PDF |
| Alternate Webpage(s) | https://www.ars.usda.gov/ARSUserFiles/35964/2009BhagwatEtAl155Microbiology229-237.pdf |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/micro/155/1/229.pdf?accname=guest&checksum=D9D0A76EE2F6ACCD597D95AE84437B8A&expires=1540289340&id=id |
| PubMed reference number | 19118363v1 |
| Alternate Webpage(s) | https://doi.org/10.1099/mic.0.023747-0 |
| DOI | 10.1099/mic.0.023747-0 |
| Journal | Microbiology |
| Volume Number | 155 |
| Part | 1 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Acids Adenosine Triphosphate Anabolism Clinical act of insertion Glucans Glucose Insertion Mutation Kanamycin Resistance Lethal Dose 50 Lipopolysaccharides Operon Osmolarity Quantity Salmonella enterica subsp. enterica serovar enteritidis Serotype Virulence periplasm |
| Content Type | Text |
| Resource Type | Article |
