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Cytotoxicity, Regulation of Apoptotic and Anti-apoptotic Gene Expression by IL-27 in MCF-7 and MDA-MB-231 Breast Cancer Cell Lines
| Content Provider | Semantic Scholar |
|---|---|
| Author | Boon, Yap Wei Nadarajah, Shaktypreya Shidik, Nadiah Alitheen, Noorjahan Banu Mohammed |
| Copyright Year | 2018 |
| Abstract | Breast cancer is one of the commonest cancers among women. Conventional therapies cause adverse side effects in patients. Cytokine immunotherapy such as interleukin-27 (IL-27) has been sought as an alternative cancer treatment in recent years. IL-27 has been shown to improve anticancer immunity and anti-angiogenesis in cancers, however, its effect on apoptotic and anti-apoptotic gene expression especially in breast cancers is yet to be explored. Cytotoxicity of IL-27 in non-cancerous (184b5) and cancerous breast cell lines was first determined for 24-72 h in this study. The results indicated that IL-27 treatment did not retard 184b5 cell growth, however, did inhibit MCF-7 (48 h) and MDAMB-231 (72 h) cell growth with IC50 at 442 and 457 ng/ml, respectively. Apoptotic (TRAIL, FADD, FAS, caspase-3 and caspase-8) and anti-apoptotic (BCL-2, AKT, and COX-2) genes were then amplified from untreated (control) and treated breast cancer cells and studied. TRAIL, caspase-3, caspase-8 gene expression was significantly (p < 0.05) upregulated in treated MCF-7 (442 ng/ml) and MDA-MB-231 (457 ng/ml) cells. Expression of FADD and FAS genes was not detected in both control and treated MCF-7 and MDA-MB-231 cells. COX-2 gene was also not expressed by MCF-7 cells, but reduced significantly (p < 0.05) in treated MDA-MB-231 cells. In MDA-MB-231 cells, IL-27 treatment seemed to slightly enhance the expression of AKT and BCL-2 genes which, on the other hand, was downregulated in treated MCF-7 cells. Conclusively, IL-27 is able to inhibit breast cancer cell growth and regulate apoptotic and anti-apoptotic gene expression in breast cancer cells. DOI : http://dx.doi.org./10.17576/JSKM-2018-03 |
| Starting Page | 15 |
| Ending Page | 22 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.17576/jskm-2018-16si-03 |
| Volume Number | 16 |
| Alternate Webpage(s) | http://ejournal.ukm.my/jskm/article/download/23981/7890 |
| Alternate Webpage(s) | https://doi.org/10.17576/jskm-2018-16si-03 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
