NDLI: Bubble cell for magnetic bead trapping in capillary electrophoresis

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Bubble cell for magnetic bead trapping in capillary electrophoresis

Content Provider	Semantic Scholar
Author	Gassner, Anne-Laure Proczek, Gaëlle Girault, Hubert H.
Copyright Year	2011
Abstract	AbstractA bubble cell capillary classically used to extend the optical path length for UV–vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case with magnetic beads trapped in a standard capillary, thereby having less effect on the experimental conditions. Using numerical simulations and microscopic visualizations, the capture of beads inside a bubble cell was investigated with two magnet configurations. Pressure-driven and electro-osmotic flow velocities were measured for different amounts of protein-A-coated beads or C18-functionalized beads (RPC-18). Solid-phase extraction of a model antibody on protein-A beads and preconcentration of fluorescein on RPC-18 beads were performed as proof of concept experiments. FigureIsovalues of the magnetic induction produced by two permanent magnets in attraction configuration with a capillary placed between them.
Starting Page	276
Ending Page	280
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://infoscience.epfl.ch/record/170704/files/216_2011_5417_MOESM1_ESM.pdf
Alternate Webpage(s)	https://infoscience.epfl.ch/record/170704/files/art3A10.10072Fs00216-011-5417-1.pdf
PubMed reference number	21964748v1
Alternate Webpage(s)	https://doi.org/10.1007/s00216-011-5417-1
DOI	10.1007/s00216-011-5417-1
Journal	Analytical and bioanalytical chemistry
Volume Number	401
Issue Number	10
Language	English
Access Restriction	Open
Subject Keyword	Bead Dosage Form Blood capillaries Capillary vessel Detectors Diameter (qualifier value) Electrophoresis Electrophoresis, Capillary Fluorescein Magnet Plug (physical object) analyte voltage
Content Type	Text
Resource Type	Article

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