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A common mechanism underlies stretch activation and receptor activation of TRPC6 channels.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hewavitharana, Thamara Xu, Wen Rong Soboloff, Jonathan Gill, Donald L. |
| Copyright Year | 2006 |
| Abstract | The TRP family of ion channels transduce an extensive range of chemical and physical signals. TRPC6 is a receptor-activated nonselective cation channel expressed widely in vascular smooth muscle and other cell types. We report here that TRPC6 is also a sensor of mechanically and osmotically induced membrane stretch. Pressure-induced activation of TRPC6 was independent of phospholipase C. The stretch responses were blocked by the tarantula peptide, GsMTx-4, known to specifically inhibit mechanosensitive channels by modifying the external lipid-channel boundary. The GsMTx-4 peptide also blocked the activation of TRPC6 channels by either receptor-induced PLC activation or by direct application of diacylglycerol. The effects of the peptide on both stretch- and diacylglycerol-mediated TRPC6 activation indicate that the mechanical and chemical lipid sensing by the channel has a common molecular mechanism that may involve lateral-lipid tension. The mechanosensing properties of TRPC6 channels highly expressed in smooth muscle cells are likely to play a key role in regulating myogenic tone in vascular tissue. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/103/44/16586.full.pdf |
| PubMed reference number | 17056714v1 |
| Volume Number | 103 |
| Issue Number | 44 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 1,2-diacylglycerol Blood Vessel Tissue Cations GsMTx-4 Ion Channel Iontophoresis Muscle Cells Muscle, Smooth, Vascular Myocytes, Smooth Muscle Phospholipase C Receptor Activation Process Smooth muscle (tissue) TRPC6 gene Tension Tissue membrane Tryptophan |
| Content Type | Text |
| Resource Type | Article |
