NDLI: Polymerase chain reaction and restriction fragment length polymorphism analysis of varicella-zoster virus isolates from the United States and other parts of the world.

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Polymerase chain reaction and restriction fragment length polymorphism analysis of varicella-zoster virus isolates from the United States and other parts of the world.

Content Provider	Semantic Scholar
Author	Larussa, Philip S. Steinberg, Sharon Arvin, Ann H. Burgess, Margaret Rekrut, K. Yamanishi, Koichi
Copyright Year	1998
Abstract	A polymerase chain reaction (PCR) assay that identifies and differentiates wild-type (wt) and vaccine strains of varicella-zoster virus (VZV) was used to determine if VZV strains with restriction fragment length polymorphisms resembling those of the Japanese Oka vaccine strain were present in the wt pool outside of Japan. Virus samples (n = 114) from patients with chickenpox and zoster from various parts of the United States and Australia were analyzed. The assay correctly identified 113 samples as wt strain. The 1 sample identified as Oka vaccine strain came from a child with leukemia who developed a vaccine-associated rash after receiving the live attenuated varicella vaccine. At this point, there is no evidence that wt strains resembling the vaccine are circulating outside of Japan. This indicates that this PCR assay can be utilized to distinguish rashes due to vaccine and wt VZV.
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Alternate Webpage(s)	https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/jid/178/Supplement_1/10.1086/514267/2/178-Supplement_1-S64.pdf?Expires=1492745850&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=DuX-rAdfRj1Gny30xQ7ALYMJe8FOQrCmW3tWN6voHD5d4BlEwrN1GnmCGOBz4BYUJDmscC7WLqaCPpQHPTrAyrhjkD9eCC38wwrdAHSvSXAs3lpE6a--RRZN3GWSOKz-Gl~PtWwl3MQCa0JgCHKBzofPMWf2~pm7Fwqa2xjh~uwH8e6nygI-L1pxwOCsASOb2pjoYNVsNjE-6gn5OaIW43BvRJGvEafgiPO-LFUOW86lZrRFvqPmcHzMcdTOYfx9L7~R3zMJYI2q6nLMCQtzMW~yUjSHhtVefC2jZ3rvw5sI5~zOeO3eyEAJaQdeVBk1j8PsuMcq~ZXRGL45Uv9vfg__
PubMed reference number	9852977v1
Volume Number	178
Journal	The Journal of infectious diseases
Language	English
Access Restriction	Open
Subject Keyword	Chickenpox Vaccine Exanthema Genetic Polymorphism Herpes Zoster Vaccine Herpes zoster disease Human herpesvirus 3 Murine sarcoma viruses Okadaic Acid Patients Polymerase Chain Reaction Restriction fragment length polymorphism leukemia varicella-zoster immune globulin
Content Type	Text
Resource Type	Article

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