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A recessive mutant of Drosophila Clock reveals a role in circadian rhythm amplitude.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Allada, Ravi Kadener, Sebastián Nandakumar, Namrata Rosbash, Michael |
| Copyright Year | 2003 |
| Abstract | The transcription factor Clock (Clk) plays a critical role in animal circadian rhythms. Genetic studies defining its function have relied on two dominant negative alleles, one in Drosophila and one in mice. Here we describe a novel recessive allele of Drosophila Clock, Clk(ar). Homozygous Clk(ar) flies are viable and behaviorally arrhythmic. The Clk(ar) phenotype is caused by a splice site mutation that severely disrupts splicing and reduces Clk activity. Despite the behavioral arrhythmicity, molecular oscillations are still detectable in Clk(ar) flies. Transcription analysis indicates potent effects of Clk(ar) on levels and amplitude of transcriptional oscillations. Taken together with other data, we propose that Clk makes a major contribution to the strength and amplitude of circadian rhythms. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bio.brandeis.edu/rosbash-lab/PublishedPapers/2003-2007/Allada-EMBO-2003.pdf |
| Alternate Webpage(s) | http://www.bio.brandeis.edu/rosbashlab/PublishedPapers/2003-2007/Allada-EMBO-2003.pdf |
| PubMed reference number | 12839998v1 |
| Volume Number | 22 |
| Issue Number | 13 |
| Journal | The EMBO journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CLOCK gene Circadian Rhythms Diptera Dominant-Negative Mutation Homozygote RNA Splicing Sleep Disorders, Circadian Rhythm TRANSCRIPTION FACTOR Transcription, Genetic |
| Content Type | Text |
| Resource Type | Article |
