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Latency-associated transcripts of equine herpesvirus type 4 in trigeminal ganglia of naturally infected horses.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Borchers, Kerstin Ludwig, Holger |
| Copyright Year | 1999 |
| Abstract | Equine herpesvirus type 4 (EHV-4) is a major respiratory pathogen of horses. Unlike most other members of the Alphaherpesvirinae, EHV-4 was regarded as non-neurotropic. Here, neural and lymphoid tissues of 17 horses have been analysed post-mortem. EHV-4 DNA was detected in 11 cases (65%) by PCR, exclusively in the trigeminal ganglia. In order to define the transcriptional activity, RNA preparations of 10 EHV-4 DNA-positive ganglia were investigated by nested RT-PCR. EHV-4-specific transcripts derived from genes 63 [herpes simplex virus type 1 (HSV-1) ICPO gene homologue] and 64 (HSV-1 ICP4 gene homologue) were detected in six trigeminal ganglia. In one other case, only gene 64-specific transcripts were present. All of the transcripts proved to be antisense orientated when a strand-specific RT-PCR was applied. Type-specific primers for gene 33 (encoding glycoprotein B) served to detect transcripts of an acute EHV-4-infection, which were found in only one of the six ganglia positive for gene 63- and gene 64-specific transcripts. Overall, these studies clearly demonstrate that EHV-4 is latent in trigeminal ganglia. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://vir.sgmjournals.org/content/80/8/2165.full.pdf |
| Alternate Webpage(s) | https://pdfs.semanticscholar.org/268d/0585e04711dc9bc730f13a4084c39c6fbeae.pdf |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/80/8/0802165a.pdf?accname=guest&checksum=D4742CCA7C3647A0F37E6F13CDF42653&expires=1539521414&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/80/8/0802165a.pdf?accname=guest&checksum=C4A6984AFE09BD2DEE37EF77BB084C70&expires=1544905954&id=id |
| PubMed reference number | 10466816v1 |
| Volume Number | 80 |
| Part | 8 |
| Journal | The Journal of general virology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Alphaherpesvirinae Body tissue Equine herpesvirus Ganglia Herpes Simplex Infections Herpesviridae Herpesvirus 4, Equid Homologous Gene Human herpesvirus 1 Lymphoid Tissue Pathogenic organism RNA Reverse Transcriptase Polymerase Chain Reaction Structure of trigeminal ganglion Transcript Transcription, Genetic antisense therapy |
| Content Type | Text |
| Resource Type | Article |
