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Callus induction and plantlet regeneration in Bixa oreliana L., an annatto-yielding tree

Content Provider Semantic Scholar
Author Khan, P. S. Sha Valli Prakash, Ekambaranellore
Copyright Year 2007
Abstract SummaryA protocol has been developed for plantlet regeneration from seed callus of Bixa orellana L. Seeds demonstrated a high percentage of callus induction (63±7.3%) and a high yield (356±14.7 mg per seed) of white friable callus on Murashige and Skoog (MS) medium containing 5.0 μM l-naphthaleneacetic acid (NAA) and 2.5μM N6-benzyladenine (BA) within 6 wk of culture in the dark. Callus induction frequency was greater under 24h dark as compared to 16h light/8h dark photoperiod or 24h light photoperiod. Increased myo-inositol (MI: 200mgl−1) and addition of ascorbic acid (AA: 200 mgl−1) to the culture medium positively improved callus induction frequency and growth. Shoot differentiation from white friable seed callus was best using 10.0 μM BA and 5.0 μM NAA, where the highest percentage of calluses forming shools (74.9±4.8%), the highest number of shoots per callus (six or seven) and the highest shoot-forming index (5.0) were obtained within 6 wk. Shoots elongated to 4 cm within 4 wk of transfer onto MS medium devoid of growth regulators. Shoots were rooted using half-strength MS medium containing 5.0 μM indole-3-butyric acid (IBA). About 85% of these plants were established in pots containing pure garden soil and organic manure after 3 wk of hardening. Regenerated plants were morphologically uniform with normal leaf, shape and growth patterns. These plants are currently being screened for the presence of agronomically useful genetic variants.
Starting Page 186
Ending Page 190
Page Count 5
File Format PDF HTM / HTML
DOI 10.1079/IVP2001284
Volume Number 38
Alternate Webpage(s) https://page-one.springer.com/pdf/preview/10.1079/IVP2001284
Alternate Webpage(s) https://doi.org/10.1079/IVP2001284
Journal In Vitro Cellular & Developmental Biology - Plant
Language English
Access Restriction Open
Content Type Text
Resource Type Article

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Callus induction and plantlet regeneration in Bixa oreliana L., an annatto-yielding tree

Callus Induction and Plantlet Regeneration in Blackgram (Vigna mungo L. Hepper)

EVALUATION OF SOME GROWTH REGULATORS COMBINATION FOR CALLUS INDUCTION IN ANNATTO (Bixa orellana L.) ACTIVE PLANT AGAINST SNAKE BITES

Callus induction and plantlet regeneration in Aegle marmelos (L.) Corr. using cotyledon explants.

RETRACTED ARTICLE: In vitro clonal propagation of annatto (Bixa oreliana L.)

Induction of embryogenic callus and plantlet regeneration from young leaves of high yielding mature oil palm

Callus induction from leaves of differentpaulownia species and its plantlet regeneration

Efficient isolated microspore culture protocol for callus induction and plantlet regeneration in japonica rice (Oryza sativa L.)

In vitro Plantlet Regeneration from Callus Culture of Trachyspermum copticum

Callus induction and plantlet regeneration in Bixa oreliana L., an annatto-yielding tree

Content Provider	Semantic Scholar
Author	Khan, P. S. Sha Valli Prakash, Ekambaranellore
Copyright Year	2007
Abstract	SummaryA protocol has been developed for plantlet regeneration from seed callus of Bixa orellana L. Seeds demonstrated a high percentage of callus induction (63±7.3%) and a high yield (356±14.7 mg per seed) of white friable callus on Murashige and Skoog (MS) medium containing 5.0 μM l-naphthaleneacetic acid (NAA) and 2.5μM N6-benzyladenine (BA) within 6 wk of culture in the dark. Callus induction frequency was greater under 24h dark as compared to 16h light/8h dark photoperiod or 24h light photoperiod. Increased myo-inositol (MI: 200mgl−1) and addition of ascorbic acid (AA: 200 mgl−1) to the culture medium positively improved callus induction frequency and growth. Shoot differentiation from white friable seed callus was best using 10.0 μM BA and 5.0 μM NAA, where the highest percentage of calluses forming shools (74.9±4.8%), the highest number of shoots per callus (six or seven) and the highest shoot-forming index (5.0) were obtained within 6 wk. Shoots elongated to 4 cm within 4 wk of transfer onto MS medium devoid of growth regulators. Shoots were rooted using half-strength MS medium containing 5.0 μM indole-3-butyric acid (IBA). About 85% of these plants were established in pots containing pure garden soil and organic manure after 3 wk of hardening. Regenerated plants were morphologically uniform with normal leaf, shape and growth patterns. These plants are currently being screened for the presence of agronomically useful genetic variants.
Starting Page	186
Ending Page	190
Page Count	5
File Format	PDF HTM / HTML
DOI	10.1079/IVP2001284
Volume Number	38
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1079/IVP2001284
Alternate Webpage(s)	https://doi.org/10.1079/IVP2001284
Journal	In Vitro Cellular & Developmental Biology - Plant
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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Callus induction and plantlet regeneration in Bixa oreliana L., an annatto-yielding tree

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Callus induction and plantlet regeneration in Aegle marmelos (L.) Corr. using cotyledon explants.

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