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A novel genome-wide full-length kinesin prediction analysis reveals additional mammalian kinesins
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chuanhai, Fu Zhen, Dou Qing, Zhou Xue-Biao, Yao |
| Copyright Year | 2006 |
| Abstract | Kinesin superfamily of microtubule-based motor orchestrates a variety of cellular processes. Recent availability of mammalian genomes has enabled analyses of kinesins on the whole genome. Here we present a novel full-length kinesin prediction program (FKPP) for mammalian kinesin gene discovery based on a comparative genomics approach. Contrary to previous predictions of 94 kinesins, we identify a total of 134 potentially kinesin genes from mammalian genomes, including 45 from mouse, 45 from rat and 44 from human. In addition, FKPP synthesizes 25 potentially full-length mammalian kinesins based on the partial sequences in the database. Surprisingly, FKPP reveals that full-length human CENP-E contains 2701 aa rather than 2663 aa in the database. Experimentation using sequence specific antibody and cDNA sequencing of human CENP-E validates the accuracy of FKPP. Given the remarkable computing efficiency and accuracy of FKPP, we reclassify the mammalian kinesin superfamily. Since current databases contain many incomplete sequences, FKPP may provide a novel approach for molecular delineation of kinesins and other protein families. |
| Starting Page | 1836 |
| Ending Page | 1847 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11434-006-2054-8 |
| Volume Number | 51 |
| Alternate Webpage(s) | http://biocuckoo.org/pub/FKPP_CSB_en.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/s11434-006-2054-8 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
