Determination of Organochlorine Compounds in Human Adipose Tissue Using Automated Liquid Chromatographic Clean Up and Gas Chromatography-Tandem Mass Spectrometry

Content Provider	Semantic Scholar
Author	Guerrero
Abstract	A multiresidue method based on automated normal-phase LC clean-up and GC-MS-MS analysis has been developed for the determination of organochlorine pesticides and polychlorinated biphenyls in human adipose tissues. Analytes were extracted by dissolving the fat samples in n-hexane, and the hexane extracts were injected onto a silica gel column for automated LC clean-up prior to analysis by GC-MS-MS. The high efficiency of the HPLC clean up for the elimination of fats in combination and the use of GC with tandem mass spectrometry detection gave detection limits at the low ng g 1 concentration level for all analytes using as little as 0.25 g of sample. The overall analytical procedure was applied to several human abdominal fat tissue samples: HCB, 13-HCH, p,p'-DDE and the higher chlorinated PCBs (138, 153 and 180) were the most frequently detected compounds, at levels of 2 6 1273 ng g 1, 19 -933 ng g 1,121 3945 ng g 1 and 5 6 4 9 6 ng g 1, respectively.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1007/BF02491787
Language	English
Access Restriction	Open
Subject Keyword	Abdominal Fat Adipose tissue C10orf90 gene Dichlorodiphenyl Dichloroethylene Excretory function Extraction File Allocation Table Flatulence Heterocyclic Compounds, 2-Ring Hexachlorobenzene Pesticides Platelet Glycoprotein 4, human Polychlorinated Biphenyls Silica Gel Silicon Dioxide Tandem Mass Spectrometry analyte n-hexane
Content Type	Text
Resource Type	Article