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Heterologous production of functional forms of Rhizopus oryzae lipase in Escherichia coli.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lorenzo, Mirella Di Hidalgo, Aurelio Haas, Michael P. Bornscheuer, Uwe T. |
| Copyright Year | 2005 |
| Abstract | To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems. |
| File Format | PDF HTM / HTML |
| DOI | 10.1128/AEM.71.12.8974-8977.2005 |
| PubMed reference number | 16332903 |
| Journal | Medline |
| Volume Number | 71 |
| Issue Number | 12 |
| Alternate Webpage(s) | https://naldc.nal.usda.gov/download/19976/PDF |
| Alternate Webpage(s) | https://doi.org/10.1128/AEM.71.12.8974-8977.2005 |
| Journal | Applied and environmental microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |