Semi-nondestructive genetic sampling from live eusocial wasps, Polistes dominulu and Polistes fuscatu

Content Provider	Semantic Scholar
Author	Starks, Philip T. Peters, J. M. H.
Copyright Year	2002
Abstract	Summary: We present a method of extracting DNA from medium-sized paper wasps without sacrificing the animal. From the distal half of a single leg, enough DNA is extracted for 125 PCR reactions. This DNA - collected from Polistes dominulus and P. fuscatus– amplifies as well as DNA extracted from larger amounts of tissue taken from sacrificed animals, and the resulting microsatellite regions amplify faithfully. Although the nest tenure of P. dominulus workers with leg-cuts was shorter than control animals, test animals appeared to behave normally. This DNA extraction method is well suited for studies that combine behavioral observations and genetic analysis on animals likely to disappear during the observation period. Collecting genetic data without destroying colonies will enable researchers to examine behavior in eusocial insects without damaging the future population size or altering the population genetic structure.
Starting Page	20
Ending Page	22
Page Count	3
File Format	PDF HTM / HTML
DOI	10.1007/s00040-002-8272-4
Volume Number	49
Alternate Webpage(s)	http://ase.tufts.edu/biology/labs/starks/publications/PDF/12.StarksPeters2002.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s00040-002-8272-4
Journal	Insectes Sociaux
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article